Comprehensive comparative study of eco-friendly Univariate and multivariate methodological approaches on processing
Yasmin M Fayez1, Adel M Michael2, Hany H Monir1
1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|September 5, 2020
Summary
This study introduces eco-friendly spectrophotometric methods for analyzing four veterinary drugs, including sulphadimidine sodium (SDS) and vitamin K3 (VTK3), without sample pretreatment. These validated green methods offer accurate and efficient quality control analysis.
Area of Science:
- Analytical Chemistry
- Green Chemistry
- Spectrophotometry
Background:
- Accurate quantification of veterinary drugs like sulphadimidine sodium (SDS), sulphaquinoxaline sodium (SQS), diaveridine (DVD), and vitamin K3 (VTK3) is crucial for quality control.
- Existing analytical methods may involve complex sample preparation or hazardous reagents, prompting the need for greener alternatives.
Purpose of the Study:
- To develop and compare eco-friendly spectrophotometric methods for the simultaneous analysis of a quaternary mixture of SDS, SQS, DVD, and VTK3.
- To validate these green analytical approaches for routine quality control applications.
Main Methods:
- Development of univariate spectrophotometric methods: successive ratio subtraction coupled with constant multiplication (SRS-CM), absorbance subtraction (AS), and amplitude modulation (AM).
- Development of multivariate spectrophotometric models using principal component regression (PCR) and partial least-squares (PLS).
- All methods were validated and utilized water as the sole reagent, ensuring environmental friendliness.
Main Results:
- The proposed univariate and multivariate spectrophotometric methods demonstrated linearity over specified concentration ranges for each drug.
- Specificity was confirmed through analysis of laboratory-prepared mixtures and a combined dosage form.
- Statistical comparison (Student's t-test and F-test) with official/reported methods showed no significant difference, indicating high accuracy and reliability.
Conclusions:
- The developed eco-friendly spectrophotometric methods are effective for the simultaneous determination of SDS, SQS, DVD, and VTK3 in mixtures.
- These green analytical techniques are suitable for routine quality control in pharmaceutical and veterinary laboratories.
- The study highlights the potential of simple, water-based spectrophotometric approaches for complex mixture analysis.
Keywords:
Absorbance subtractionAmplitude modulationMultivariate greener models, veterinary formulationSuccessive ratio subtractionMore Related Videos
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