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Fluorescent In Situ Hybridization and 5-Ethynyl-2'-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum
Published on: August 3, 2022
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Pattern regulation in a regenerating jellyfish
Chiara Sinigaglia1, Sophie Peron1, Jeanne Eichelbrenner1
1Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Villefranche-sur-mer, France.
Elife
|September 7, 2020
Summary
Jellyfish regeneration rapidly restores shape and function through mechanical forces and cell movements. Local interactions guide patterning, particularly for the central feeding organ, highlighting a novel regenerative mechanism.
Area of Science:
- Developmental Biology
- Regenerative Medicine
- Marine Biology
Background:
- Jellyfish possess tetraradial symmetry, offering unique insights into biological patterning.
- Understanding regeneration mechanisms is crucial for regenerative medicine and developmental biology.
Purpose of the Study:
- To investigate the mechanisms underlying rapid regeneration in jellyfish fragments.
- To elucidate the roles of mechanical forces, cell behaviors, and signaling pathways in restoring form and function.
Main Methods:
- Fragmentation of jellyfish specimens.
- Analysis of actomyosin-based remodeling and cell migration.
- Gene expression analysis (Wnt6).
- Investigation of Wnt/β-catenin signaling pathway.
Main Results:
- Jellyfish fragments rapidly restore shape and functionality, including the central feeding organ (manubrium).
- Actomyosin-powered remodeling and cell convergence around 'hubs' establish positional landmarks.
- Wnt6 expression and stabilization of hubs depend on muscle fiber configuration.
- Manubrium regeneration is Wnt/β-catenin dependent, involving proliferation and cell recruitment.
Conclusions:
- Jellyfish regeneration relies on an interplay of mechanical forces, cell migration, and proliferation.
- Local interactions, driven by remodeling, are key to body patterning during regeneration.
- The study reveals a novel paradigm for regeneration, applicable to understanding complex biological patterning.
Keywords:
Clytia hemisphaericaCnidariaHydrozoadevelopmental biologyjellyfishmedusaregenerationregenerative medicinestem cellsMore Related Videos
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