Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications

Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying. Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm. The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability. X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film. The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay. Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold. The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.