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Generation of Human Kidney Tubuloids from Tissue and Urine
Published on: April 16, 2021
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Human kidney organoids produce functional renin
Anusha S Shankar1, Zhaoyu Du1, Hector Tejeda Mora1
1Department of Internal Medicine, Division of Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Kidney International
|September 12, 2020
Summary
Human kidney organoids derived from stem cells can produce renin, a key hormone for blood pressure regulation. These organoids offer a novel model for studying kidney physiology and endocrine functions.
Area of Science:
- Nephrology
- Stem Cell Biology
- Endocrinology
Background:
- Renin is crucial for blood pressure and salt balance.
- Lack of human models hinders renin regulation research.
- Human induced pluripotent stem cell-derived kidney organoids offer a potential solution.
Purpose of the Study:
- To develop and characterize human kidney organoids for studying renin production.
- To investigate the regulation of renin and the renin-angiotensin system in these organoids.
- To assess the endocrine potential and therapeutic applications of kidney organoids.
Main Methods:
- Utilized human induced pluripotent stem cells to generate kidney organoids.
- Performed extensive characterization including single-cell sequencing and immunohistochemistry.
- Conducted functional assays to assess renin synthesis and secretion.
- Implanted organoids in immunodeficient mice to evaluate vascularization and structural integrity.
Main Results:
- Organoids exhibited kidney-specific cell populations (podocytes, tubular cells, etc.).
- Identified renin-angiotensin system components within the organoids.
- Demonstrated that cyclic AMP stimulation induces pericytes to produce active renin.
- Showed renin production was regulated by parathyroid hormone.
- Implanted organoids maintained structure and renin production for two months in vivo.
Conclusions:
- Human kidney organoids successfully model renin production and regulation.
- These organoids possess endocrine potential relevant for regenerative medicine.
- The developed model provides new avenues for studying kidney physiology and diseases.
Keywords:
cyclic AMPinduced pluripotent stem cellskidney organoidsparathyroid hormonepericyterenin-angiotensin systemMore Related Videos
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