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Updated: Dec 9, 2025

Mating-based Overexpression Library Screening in Yeast
Published on: July 6, 2018
FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae
Gowsalya Ramachandran1, Ravi Chidambaram1, Vasanthi Nachiappan2
1Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
Objectives:
To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis.
Results:
Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1Δ cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae.
Conclusions:
These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS).
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