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Updated: Dec 9, 2025

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.
Published on: September 1, 2017
A Simple and Effective Method for Speciation Analysis of 13 Arsenic Species Using HPLC on a Fluorocarbon Stationary
Shin-Ichi Miyashita1, Kenji Kinoshita2, Toshikazu Kaise3
1Environmental Standards Group, Research Institute for Material and Chemical Measurement, National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 3, 1-1-1 Umezono Tsukuba, Ibaraki, 305-8563, Japan. shinichi-miyashita@aist.go.jp.
Abstract:
The separation properties of arsenic species was investigated using HPLC-ICP-MS with several commercially available fluorocarbon stationary phases and no ion-pair reagents in HPLC. One pentafluorophenyl column showed the highest potential for the separation of a larger number of arsenic species when using simple acid-based mobile phases. After modification of the operational parameters in HPLC, the speciation analysis of 13 representative arsenic species: arsenite, arsenate, methylarsonic acid, dimethylarsinic acid, trimethylarsine oxide, tetramethylarsonium, arsenobetaine, arsenocholine, thio-dimethylarsinic acid, oxo-arsenosugar-glycerol, oxo-arsenosugar-phosphate, oxo-arsenosugar-sulfonate, and oxo-arsenosugar-sulfate, was achieved by HPLC-ICP-MS with the column along with a mobile phase of 0.05% heptafluorobutyric acid-methanol (99:1, volume per volume).
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