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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Published on: August 9, 2022
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Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules
Fatemeh Shafiei1, Kathleen McAuliffe1, Yousef Bagheri1
1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Analytical Methods : Advancing Methods and Applications
|September 15, 2020
Summary
This study introduces a novel paper-based sensor using fluorogenic RNA aptamers for rapid point-of-care testing. The affordable system enables sensitive detection of various targets like antibiotics and signaling molecules.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Sensor Technology
Background:
- Fluorogenic RNA aptamer sensors offer versatile in vitro and intracellular detection capabilities.
- The application of these RNA sensors in point-of-care testing (POCT) remains underexplored.
Purpose of the Study:
- To develop and evaluate a portable, paper-based fluorogenic RNA sensor system for point-of-care applications.
- To demonstrate the system's capability for selective, sensitive, and rapid detection of diverse analytes.
Main Methods:
- Embedding fluorogenic RNA sensors into a paper substrate.
- Utilizing a simple rehydration process for target analyte detection.
Main Results:
- The paper-based sensor system allows for straightforward target detection upon rehydration.
- The system demonstrated selective, sensitive, and rapid detection of various analytes, including antibiotics and cellular signaling molecules.
Conclusions:
- Paper-based fluorogenic RNA sensors represent a promising, affordable platform for point-of-care testing.
- This technology has broad potential for detecting a wide range of targets, from small molecules to pathogens.
