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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Gas Chromatography: Overview of Detectors01:13

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Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
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Detection challenges in quantitative polymer analysis by liquid chromatography.

Wouter C Knol1,2, Bob W J Pirok1,2, Ron A H Peters1,2,3

  • 1Analytical Chemistry Group, van't Hoff Institute for Molecular Sciences (HIMS), Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands.

Journal of Separation Science
|September 16, 2020
PubMed
Summary
This summary is machine-generated.

Accurate polymer quantification in liquid chromatography is difficult due to detector responses varying with polymer composition. This review explores advanced detection methods for analyzing complex polymers without standards.

Keywords:
detectionliquid chromatographypolymersquantification

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Area of Science:

  • Polymer Chemistry
  • Analytical Chemistry
  • Chromatography

Background:

  • Accurate quantification of polymer distributions is a major challenge in polymer analysis via liquid chromatography.
  • Detector responses are influenced by polymer characteristics like molecular weight, composition, and branching, complicating analysis, especially for polymers lacking standards.
  • Mobile-phase composition changes can further limit detection technique applicability.

Purpose of the Study:

  • To review current and emerging detection methods for liquid chromatography.
  • To identify detectors suitable for the quantitative analysis of complex polymers.

Main Methods:

  • Literature review of existing and novel detection techniques in liquid chromatography.
  • Analysis of detector responses in relation to polymer compositional features.

Main Results:

  • Current detectors face limitations in accurately quantifying complex polymers due to variable responses and lack of standards.
  • Refractive index detection is often inaccurate for complex samples due to unknown refractive-index increments.

Conclusions:

  • There is a need for advanced detection methods in liquid chromatography for accurate polymer quantification.
  • Identifying suitable detectors is crucial for advancing the analysis of complex polymer systems.