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Related Concept Videos

Long-patch Base Excision Repair01:02

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Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
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One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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Following the Dynamics of Structural Variants in Experimentally Evolved Populations
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Base Editing Landscape Extends to Perform Transversion Mutation.

Kutubuddin A Molla1, Yiping Qi2, Subhasis Karmakar1

  • 1Indian Council of Agricultural Research (ICAR)-National Rice Research Institute (NRRI), Cuttack-753006, India.

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|September 21, 2020
PubMed
Summary
This summary is machine-generated.

New base editors can now install transversion mutations, expanding the precision gene editing toolbox. These cytosine transversion base editors broaden the scope of DNA base editing applications.

Keywords:
C-to-G editingCRISPR/Casagriculturebase editingpoint mutationtherapeuticstransversion base editor

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Base editors precisely alter single DNA bases, attracting significant research and industrial interest.
  • Current cytosine and adenine base editors are limited to installing transition mutations.

Purpose of the Study:

  • To expand the capabilities of base editing technology.
  • To develop novel base editors capable of installing transversion mutations.

Main Methods:

  • Development of new cytosine base editors.
  • Testing the efficiency and specificity of the novel editors.

Main Results:

  • Successful development of cytosine transversion base editors.
  • Demonstration of the ability to install specific transversion mutations.

Conclusions:

  • The new cytosine transversion base editors significantly broaden the base editing toolbox.
  • These advancements offer expanded possibilities for genetic research and therapeutic applications.