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DNAzyme-gold nanoparticle-based probes for biosensing and bioimaging
Ling Hu1, Xiaoyi Fu1, Gezhi Kong1
1Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. glke@hnu.edu.cn xbzhang@hnu.edu.cn.
Journal of Materials Chemistry. B
|September 21, 2020
Summary
Deoxyribozymes (DNAzymes), catalytic DNA molecules, combined with gold nanoparticles offer enhanced biosensing and bioimaging capabilities. This review explores their structure, preparation, and diverse applications in recent years.
Area of Science:
- Biochemistry and Molecular Biology
- Nanotechnology and Materials Science
- Analytical Chemistry and Sensor Technology
Background:
- Deoxyribozymes (DNAzymes) are catalytic nucleic acids with programmable activity, stability, and excellent catalytic efficiency.
- Gold nanoparticles possess unique photoelectric properties and can enhance DNAzyme performance, including cell transfection and nuclease resistance.
- DNAzyme-gold nanoparticle complexes are emerging as powerful tools for biosensing and bioimaging applications.
Purpose of the Study:
- To provide a comprehensive overview of DNAzyme structure, catalytic activity, and preparation methods for DNAzyme-gold nanoparticle conjugates.
- To review recent advancements (past five years) in the application of DNAzyme-gold nanoparticle probes for biosensing and bioimaging.
- To discuss the challenges and future opportunities in the field of DNAzyme-gold nanoparticle-based technologies.
Main Methods:
- Introduction to DNAzyme structure, catalytic mechanisms, and coenzyme-dependent activity.
- Overview of various methods for synthesizing and functionalizing DNAzyme-gold nanoparticle complexes.
- Categorization of DNAzyme-gold nanoparticle sensors based on output signals: fluorescence, colorimetric, electrochemical, and photoelectrochemical.
Main Results:
- DNAzyme-gold nanoparticle probes demonstrate significant potential in diverse biosensing applications, including environmental monitoring and food safety.
- These complexes are effective in bioimaging, leveraging their unique optical and catalytic properties.
- Recent studies highlight the development of sensitive and selective sensors based on fluorescence, colorimetric, and electrochemical detection principles.
Conclusions:
- DNAzyme-gold nanoparticle complexes represent a highly promising platform for advanced biosensing and bioimaging due to their synergistic properties.
- Continued research is expected to overcome current challenges, leading to novel applications in diagnostics and therapeutic monitoring.
- The field offers significant opportunities for innovation in developing next-generation analytical tools and imaging agents.
