[Establishment of a mouse neutrophil-dominated house dust mite allergic asthma model]

Objective To establish a mouse neutrophil-dominated asthma model using house dust mite (HDM). Methods With a random number table, a total of 16 specific pathogen-free (SPF) BALB/c mice were divided into control group and model group. The model group was sensitized and stimulated by HDM nasal drip, and the control group mice were given the same amount of saline. In 24 hours after the last stimulation, the enhanced pause (Penh) was measured by the BUXCO noninvasive lung function detector to determine the airway resistance; the bronchoalveolar lavage fluid (BALF) was collected to count cells and classify inflammatory cells. The infiltration degree of inflammatory cells in the lung tissue was observed by HE staining. The expression of IL-6, IL-17F and IL-1β mRNA was detected by real-time quantitative PCR, and the levels of IL-4, IL-6, IL-17F and tumor necrosis factor α (TNF-α) in BALF were detected by cytometric bead array (CBA). Results Compared with the control group, the value of Penh in the model group was significantly higher; the tracheal and paravascular inflammation significantly aggravated; the levels of IL-6, IL-17F, IL-1β mRNA, the cell count and neutrophil count in BALF, and the levels of neutrophil cytokines IL-6, IL-17F and TNF-α in the BALF increased significantly. Conclusion The mouse neutrophil-dominated house dust mite allergic asthma model has been successfully established.