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Updated: Dec 8, 2025

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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
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Circularized blocker-displacement amplification for multiplex detection of rare DNA variants
Wei Lai1, Mingshu Xiao1, Haihong Yang1
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China. peihao@chem.ecnu.edu.cn.
Abstract:
A superselective isothermal amplification technique, termed circularized blocker-displacement amplification, was developed for multiplex analysis of rare DNA variants.

