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Self-Assembly of a Dual-Targeting and Self-Calibrating Ratiometric Polymer Nanoprobe for Accurate Hypochlorous Acid
Hui Liu1,2, Peisheng Zhang1,2, Chonghua Zhang2
1State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
ACS Applied Materials & Interfaces
|September 24, 2020
Summary
A novel polymer nanoprobe (SPN) enables precise lysosomal imaging of hypochlorous acid (HClO) in hepatoma cells. This dual-targeting probe offers accurate molecular imaging for improved hepatoma diagnosis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Cell Biology
Background:
- Accurate molecular imaging of hepatoma cells, particularly targeting lysosomes, is crucial for diagnosis.
- Hypochlorous acid (HClO) is a biomarker implicated in cellular processes and disease states.
Purpose of the Study:
- To develop a single-dye-based polymer nanoprobe (SPN) for dual-targeting and self-calibrating ratiometric imaging of HClO in HepG2 cell lysosomes.
- To assess the SPN's performance characteristics, including targeting accuracy, response time, stability, and biocompatibility.
Main Methods:
- Fabrication of the SPN via self-assembly, incorporating a ratiometric pyrene derivative fluorophore.
- Utilizing galactose as a hepatocyte-targeting moiety and controlling nanoparticle size/charge for lysosomal localization.
- Evaluating SPN performance in HepG2 cells, focusing on HClO detection in lysosomes.
Main Results:
- The SPN demonstrated high water dispersibility, ultrafast response (<1s), selectivity, and long-term stability (>90 days).
- SPN successfully targeted HepG2 cells via asialoglycoprotein receptors and localized within lysosomes.
- Accurate ratiometric imaging of HClO within the lysosomes of HepG2 cells was achieved.
Conclusions:
- The developed SPN provides a versatile platform for precise dual-targeting and accurate molecular imaging.
- This nanoprobe shows significant potential for advancing hepatoma diagnosis through enhanced lysosomal HClO detection.
