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In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
Published on: September 23, 2021
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Biomineralized Bacterial Outer Membrane Vesicles Potentiate Safe and Efficient Tumor Microenvironment Reprogramming
Shuang Qing1,2, Chengliang Lyu1, Li Zhu3
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Bei-Er-Tiao, Beijing, 100190, P. R. China.
Advanced Materials (Deerfield Beach, Fla.)
|October 5, 2020
Summary
Bacterial outer membrane vesicles (OMVs) coated with calcium phosphate (CaP) shells reprogram the tumor microenvironment (TME). This novel approach enhances immunotherapy efficacy by neutralizing acidity and promoting anti-tumor immune responses without side effects.
Area of Science:
- Immunology
- Materials Science
- Oncology
Background:
- Solid tumors often exhibit an immunosuppressive tumor microenvironment (TME) that limits immunotherapy effectiveness.
- Bacterial outer membrane vesicles (OMVs) show potential as immunostimulants for TME modulation.
- Intravenous delivery of OMVs faces challenges like antibody-dependent clearance and toxicity.
Purpose of the Study:
- To develop a safe and effective OMV-based platform for TME reprogramming.
- To investigate the use of calcium phosphate (CaP) shells for OMV delivery.
- To evaluate the potential for combination therapies using functionalized OMVs.
Main Methods:
- OMVs were encapsulated with pH-sensitive calcium phosphate (CaP) shells.
- The CaP-shelled OMVs were assessed for their ability to reprogram the TME.
- Macrophage polarization (M2 to M1) and antitumor effects were evaluated.
- Functional components (folic acid, photosensitizers) were integrated into the CaP shells.
Main Results:
- CaP-shelled OMVs demonstrated potent TME reprogramming without adverse effects from intravenous injection.
- The pH-sensitive CaP shells neutralized acidic TME, promoting beneficial M2-to-M1 macrophage polarization.
- Functionalized CaP-shelled OMVs showed potential for synergistic effects in combination therapies.
Conclusions:
- CaP-shelled OMVs represent a promising strategy for overcoming TME-mediated immunosuppression.
- This platform offers a safe and versatile approach for enhancing cancer immunotherapy.
- The integration of functional components opens avenues for advanced combination cancer treatments.
Keywords:
bacterial outer membrane vesiclesbiomineralizationcancer immunotherapytumor microenvironment reprogrammingMore Related Videos
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