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Tracking Mechanisms of Viral Dissemination In Vivo

Raphael Gaudin1, Jacky G Goetz2

  • 1Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, 34293 Montpellier, France; Université de Montpellier, 34090 Montpellier, France.

Trends in Cell Biology
|October 7, 2020
PubMed

Insights

Studying viral dissemination in vivo is challenging, but the zebrafish model offers a transparent platform to investigate cellular mechanisms of viral spread and replication within a complex ecosystem.

Area of Science:

  • Virology
  • Cell Biology
  • Animal Models

Background:

  • Viral dissemination involves complex, multi-step processes of infection, navigation, and cellular indoctrination.
  • Modeling viral spread in vitro often fails to capture the intricate cellular ecosystem dynamics.
  • In vivo animal models are crucial but can be cumbersome to study viral dissemination at high resolution.

Purpose of the Study:

  • To discuss in vivo platforms for dissecting viral dissemination.
  • To explore cellular and molecular programs governing viral spread.
  • To highlight the advantages of the zebrafish model for studying viral dissemination.

Main Methods:

  • Review of in vivo platforms for studying viral dissemination.
  • Discussion of organoid and ex vivo alternatives.
  • Focus on the zebrafish model as a transparent in vivo system.

Main Results:

  • The zebrafish model provides a transparent window for in vivo observation.
  • It enables the unraveling of novel cellular mechanisms of viral dissemination.
  • It aids in understanding viral spread within a complex biological system.

Conclusions:

  • In vivo models, particularly zebrafish, are essential for understanding viral dissemination.
  • The zebrafish model facilitates the discovery of new cellular strategies used by viruses.
  • This approach overcomes limitations of in vitro and less transparent in vivo models.
Keywords:
animal modelsintravital imagingviruszebrafish

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