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Chronopotentiometric aptasensing with signal amplification based on enzyme-catalyzed surface polymerization.
Shuwen Liu1, Jiawang Ding, Wei Qin
1Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, P. R. China. jwding@yic.ac.cn wqin@yic.ac.cn.
Chemical Communications (Cambridge, England)
|October 8, 2020
Summary
A novel signal amplification method uses horseradish peroxidase and dopamine polymerization for sensitive aptamer-target detection via chronopotentiometry.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensors
Background:
- Aptamer-based detection offers high specificity for various targets.
- Sensitive and reliable detection methods are crucial for aptasensors.
- Chronopotentiometry provides a quantitative electrochemical readout.
Purpose of the Study:
- To develop a sensitive signal amplification strategy for aptamer-target binding detection.
- To utilize horseradish peroxidase-catalyzed dopamine polymerization on an ion-selective membrane.
- To enable sensitive chronopotentiometric detection of aptamer-target interactions.
Main Methods:
- A polymeric ion-selective membrane was functionalized.
- Horseradish peroxidase catalyzed the polymerization of dopamine on the membrane surface.
- The polymerization process served as a signal amplification step.
- Chronopotentiometry was employed to detect the aptamer-target binding event.
Main Results:
- The proposed strategy achieved sensitive detection of aptamer-target binding.
- Signal amplification was effectively realized through dopamine polymerization.
- The method demonstrated potential for quantitative analysis.
Conclusions:
- The horseradish peroxidase/dopamine polymerization system provides a robust amplification strategy.
- This approach enhances the sensitivity of chronopotentiometric aptasensor detection.
- The method holds promise for sensitive biological and chemical sensing applications.
