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Nano-localized single-cell nano-electroporation.

Tuhin Subhra Santra1, Srabani Kar2, Hwan-You Chang3

  • 1Department of Engineering Design, Indian Institute of Technology Madras, Chennai 60036, India. tuhin@iitm.ac.in santra.tuhin@gmail.com.

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|October 13, 2020
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Summary

This study introduces a novel nano-electroporation platform for precise, targeted delivery of biomolecules into single cells. The technology achieves high transfection efficiency and cell viability, offering potential for cellular therapy and diagnostics.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Efficient delivery of foreign molecules into single cells is crucial for cell biology research and therapeutic applications.
  • Existing methods often face challenges with precision, scalability, or maintaining cell viability.

Purpose of the Study:

  • To develop and demonstrate a nano-localized single-cell nano-electroporation platform for precise cargo delivery.
  • To evaluate the platform's efficiency, viability, and versatility across different cell types and biomolecules.

Main Methods:

  • Fabrication of a platform with triangular indium tin oxide (ITO) nano-electrodes featuring a 70 nm gap and 40 nm tip diameter.
  • Application of voltage between nano-electrodes to create intense electric fields for nano-localized electroporation.
  • Gentle delivery of biomolecules (dyes, QDs, plasmids) using a pressurizing pump flow.

Main Results:

  • Successful delivery of various biomolecules into different cell types using the nano-electroporation platform.
  • Achieved high transfection efficiency (>96% for CL1-0 cells) and high cell viability (∼98%).
  • Demonstrated control over delivery by varying electric field strength, pulse duration, and pulse number.

Conclusions:

  • The developed nano-localized nano-electroporation platform enables precise and efficient delivery of diverse biomolecules into single living cells.
  • The high transfection efficiency and cell viability make this technology a promising tool for advancing cellular therapy and diagnostic research.