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Updated: Dec 5, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Automated Raman based cell sorting with 3D microfluidics.

Yingkai Lyu¹, Xiaofei Yuan², Andrew Glidle³

¹Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, UK. huabing.yin@glasgow.ac.uk and Key Laboratory of the Ministry of Education on Optoelectronic Information Technology, School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China. tyang@tju.edu.cn.

|October 15, 2020

Summary

This summary is machine-generated.

This study introduces a 3D hydrodynamic focusing microfluidic system for automated Raman activated cell sorting (RACS). The novel system achieves high throughput and purity for sorting diverse cell types, advancing label-free cell analysis.

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

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Area of Science:

Biophotonics
Microfluidics
Cell Sorting Technology

Background:

Raman activated cell sorting (RACS) offers label-free analysis linking cell phenotype and genotype.
Current RACS implementation faces limitations in throughput and handling biological complexity.

Purpose of the Study:

To develop a fully automated, continuous Raman activated cell sorting system using 3D hydrodynamic focusing.
To overcome throughput and complexity limitations in current RACS platforms.

Main Methods:

A 3D printed detection chamber (1 mm3) integrated with a PDMS sorting unit, optical sensors, and collection module was engineered.
Precise cell positioning in the detection chamber minimized spectroscopic interference.
A simple "Raman window" detection system compatible with standard inverted microscopes was employed.

Main Results:

The system demonstrated stable operation (>8 hours) and high throughput for sorting cells from 1 μm bacteria to 10s μm mammalian cells.
Proof-of-concept sorting of Chlorella vulgaris and E. coli mixtures achieved 92.0% purity at 310 cells/min.
The platform exhibited facile and robust operation.

Conclusions:

The developed 3D-RACS platform provides a versatile tool for function-based flow cytometry and cell sorting.
This technology has broad applications in microbiology, biotechnology, life sciences, and diagnostics.