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Updated: Dec 5, 2025
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
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RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa.
Mani Arul Prakash1, Arumugam Kumaresan1, Manish Kumar Sinha1
1Theriogenology Laboratory, Southern Regional Station of ICAR-National Dairy Research Institute, Adugodi, Bengaluru, 560030 Karnataka, India.
Animal Reproduction Science
|October 17, 2020
Summary
Bull sperm contain RNA transcripts that play roles in early embryonic development. This study profiled these sperm transcripts, revealing their involvement in ribosome structure, translation, and key metabolic pathways, with variations observed between bulls.
Area of Science:
- Reproductive Biology
- Molecular Genetics
- Sperm Biology
Background:
- Sperm were traditionally considered transcriptionally and translationally inactive.
- Sperm contribute functionally relevant transcripts to the oocyte, influencing early development.
- Understanding sperm transcriptomics is crucial for reproductive success.
Purpose of the Study:
- To comprehensively profile and analyze sperm transcripts in Holstein Friesian x Tharparkar crossbred bulls.
- To identify key genes and pathways involved in sperm function.
- To investigate variations in sperm transcript abundance between individual bulls.
Main Methods:
- Next-Generation Ribonucleic acid sequencing (RNA-Seq) for global transcriptomic profiling.
- Bioinformatic analysis of identified transcripts.
- Validation of selected gene transcript abundance using quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR).
Main Results:
- Global transcriptomic profiling identified transcripts for 13,814 genes.
- Abundant mRNA transcripts included PRM1 and HMGB4.
- Gene ontology analysis indicated significant involvement in structural constituent of ribosomes and translation.
- Pathway enrichment revealed connections to ribosome, oxidative phosphorylation, and spliceosome pathways.
- RT-qPCR validation showed significant inter-bull variations in transcriptional abundance.
Conclusions:
- Crossbred bull sperm transcripts are significantly implicated in ribosome structure and translation.
- Identified pathways suggest a role for sperm transcripts in cellular energy metabolism and RNA processing.
- Transcriptomic variations between bulls may have implications for semen quality and fertility, warranting further investigation.
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