Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ole Behrmann1,2, Iris Bachmann1, Frank Hufert1

  • 1Institut für Mikrobiologie und Virologie, Medizinische Hochschule Brandenburg Theodor Fontane (MHB), Universitätsplatz 1, D-01968 Senftenberg, Deutschland.

Biospektrum : Zeitschrift Der Gesellschaft Fur Biologishe Chemie (GBCH) Und Der Vereinigung Fur Allgemeine Und Angewandte Mikrobiologie (VAAM)
|October 20, 2020
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D-Printed Microfluidic Chip System with Integrated Fluidic Breakers and Phaseguide Fluid Structures for Optimal Passive Mixing.

Micromachines·2026
Same author

Development of a Rapid Isothermal Assay for Detection of Adenovirus Types Important in Respiratory Infections.

Influenza and other respiratory viruses·2025
Same author

Impact of feeding strategies on the welfare and behaviour of horses in groups: An experimental study.

PloS one·2025
Same author

Detection of <i>Klebsiella pneumoniae</i> Carbapenem Resistance Genes by qPCR: Choosing the Right Method for Total DNA Extraction.

Microorganisms·2024
Same author

Infection prevention in medical education - results of a descriptive cross-sectional study in Germany.

GMS journal for medical education·2024
Same author

Rapid Isothermal Detection of Pathogenic <i>Clostridioides difficile</i> Using Recombinase Polymerase Amplification.

Analytical chemistry·2024
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
Same journal

Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)·2023
See all related articles

A new reverse transcription recombinase polymerase amplification (RT-RPA) assay offers a rapid, isothermal method for detecting SARS-CoV-2 RNA. This fast nucleic acid detection technology provides an alternative to RT-qPCR for COVID-19 diagnostics.

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Context:

  • The COVID-19 pandemic necessitates rapid and accessible nucleic acid detection methods.
  • Existing methods like RT-qPCR require specialized equipment and thermal cycling.
  • Isothermal amplification offers a potential alternative for point-of-care diagnostics.

Purpose:

  • To develop and evaluate a reverse transcription recombinase polymerase amplification (RT-RPA) assay for SARS-CoV-2 RNA detection.
  • To establish a fast, isothermal method as an alternative to RT-qPCR.
  • To enable real-time monitoring of nucleic acid amplification.

Summary:

  • Reverse transcription recombinase polymerase amplification (RT-RPA) technology was employed for SARS-CoV-2 RNA detection.
  • RPA utilizes recombination proteins and DNA polymerase for rapid DNA amplification at a constant temperature (39-42 °C).

Related Experiment Videos

  • The RT-RPA assay achieves target amplification within 10–20 minutes and allows for real-time monitoring using fluorescent probes.

    • Impact:

    • This RT-RPA assay provides a fast and simple method for SARS-CoV-2 RNA detection.
    • The isothermal nature of RT-RPA simplifies assay requirements, potentially enabling point-of-care applications.
    • This technology offers a valuable alternative to RT-qPCR for rapid molecular diagnostics during pandemics.