Circadian Variations in Cellular Composition of Lymphoid Organs in Rats with Experimental Endomyometritis

Area of Science:

• Immunology
• Chronobiology
• Cellular Biology

Background:

• Circadian rhythms influence physiological processes, including immune function.
• Disruptions in circadian rhythms are linked to various pathologies.
• The impact of experimental endomyometritis on lymphoid organ cellular composition and circadian variations remains understudied.

Purpose of the Study:

• To investigate the circadian variations in lymphoid organ cellular composition in rats with experimental endomyometritis.
• To compare these variations with those in healthy control rats.
• To assess the potential desynchronosis of the immune system due to chronic inflammation.

Main Methods:

• Female Wistar rats were used, divided into control and experimental endomyometritis groups.
• Cellular composition (CD8+ cells, CD25+ cells, lymphocytes, monocytes/macrophages) was analyzed in thymus, spleen, and lymph nodes (para-aortic, inguinal, ileal).
• Analyses were performed at two time points: 10:00 h and 20:00 h to assess circadian variations.

Main Results:

• In rats with endomyometritis, fewer parameters showed significant circadian variations in the thymus and spleen compared to controls.
• Lymph nodes exhibited altered circadian patterns: para-aortic (CD8+, CD25+ cells, monocytes/macrophages), inguinal (large/small lymphocytes, CD8+ cells), and ileal (large lymphocytes, CD8+ cells, monocytes/macrophages).
• Chronic inflammation of the uterine and vaginal mucosa correlated with immune system desynchronosis.

Conclusions:

• Experimental endomyometritis induces desynchronosis in the immune system's circadian rhythms.
• Circadian rhythms are crucial factors to consider in the diagnosis and treatment of endomyometritis.
• Immune system desynchronization may underlie disease progression and treatment efficacy.