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Aggregate Determination by Permeation Technique.

Phennapha Saokham1, Thorsteinn Loftsson2

  • 1College of Pharmacy, Rangsit University, Pathum Thani, Thailand. phennapha.s@rsu.ac.th.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2020
PubMed
Summary
This summary is machine-generated.

The permeation technique detects nano-sized molecular aggregates in solutions. This method identifies guest/host aggregate formation and determines the critical aggregation concentration (cac) for guest molecules.

Keywords:
AggregatesCritical aggregation concentration (cac)Inclusion complexesPermeation

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Area of Science:

  • Physical Chemistry
  • Supramolecular Chemistry
  • Materials Science

Background:

  • Molecular aggregation in aqueous solutions is crucial for understanding various chemical and biological processes.
  • Cyclodextrin inclusion complexes are widely studied for their host-guest chemistry applications.
  • Detecting and quantifying nano-sized aggregates, including self-aggregates and guest/host complexes, remains a challenge.

Purpose of the Study:

  • To describe the application of the permeation technique for studying molecular aggregation.
  • To demonstrate the detection of nano-sized guest/host aggregates in aqueous solutions.
  • To determine the critical aggregation concentration (cac) of guest molecules using permeation profiles.

Main Methods:

  • Utilizing a permeation technique with semipermeable membranes to analyze molecular aggregation.
  • Monitoring the permeation of guest molecules, host molecules, and their aggregates through membranes of defined pore sizes.
  • Analyzing negative deviations in permeation profiles to infer aggregate formation.

Main Results:

  • The permeation technique effectively detects the formation of nano-sized aggregates in aqueous solutions.
  • Negative deviations in permeation profiles correlate with the formation of guest/host aggregates or self-aggregates.
  • The critical aggregation concentration (cac) of a guest molecule can be determined from its permeation profile.

Conclusions:

  • The permeation technique is a valuable tool for investigating molecular aggregation phenomena.
  • This method allows for the detection and characterization of nano-sized aggregates, including cyclodextrin-based systems.
  • The determination of critical aggregation concentration provides insights into the thermodynamics of aggregate formation.