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Related Concept Videos

Transcription Factors02:16

Transcription Factors

81.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.0K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.0K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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2.3K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.8K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.8K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.2K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.2K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.1K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.1K

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Related Experiment Video

Updated: Dec 3, 2025

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

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Advances in visualizing transcription factor - DNA interactions.

Rachel M Price1,1, Marek A Budzyński1,1, Shivani Kundra1,1

  • 1Department of Biochemistry and Molecular Biology, Life Sciences Institute, The University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.

Genome
|October 28, 2020
PubMed
Summary
This summary is machine-generated.

Advanced imaging reveals transcription factor (TF) dynamics and structure, complementing molecular biology. This approach reshapes understanding of TF-DNA interactions and cell cycle regulation, particularly mitotic bookmarking.

Keywords:
dynamicsdynamiquefacteurs de transcriptionimagerieimagingstructuretranscription factors

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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Related Experiment Videos

Last Updated: Dec 3, 2025

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
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Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Transcription factors (TFs) are crucial regulators of gene transcription through specific DNA interactions.
  • Traditional molecular biology assays have provided extensive knowledge on TF-DNA binding.
  • Recent advancements in microscopy offer new avenues to study TF-DNA interactions.

Purpose of the Study:

  • To review how diverse imaging technologies provide structural and dynamic insights into TF-DNA interactions.
  • To examine the complementary role of imaging alongside molecular biology assays.
  • To highlight how advanced imaging reshaped the understanding of TF behavior during the cell cycle.

Main Methods:

  • Review of diverse imaging technologies applied to TF-DNA interactions.
  • Analysis of structural and dynamic information obtained from imaging techniques.
  • Case study on TF behavior across the cell cycle using advanced imaging.

Main Results:

  • Imaging provides critical structural and dynamic data on TF-DNA interactions.
  • Advanced imaging techniques offer complementary insights to molecular biology assays.
  • New understanding of TF behavior during the cell cycle, including mitotic bookmarking, has emerged.

Conclusions:

  • Imaging technologies are powerful tools for studying TF-DNA interactions.
  • Integrating imaging with molecular biology deepens our understanding of transcription regulation.
  • Advanced imaging has significantly advanced the study of TF dynamics and function, particularly in cell cycle contexts like mitotic bookmarking.