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Updated: Jul 30, 2026

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CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
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Unravelling the cellular emission fingerprint of the benchmark G-quadruplex-interactive compound Phen-DC3
Marco Deiana1, Jan Jamroskovic, Ikenna Obi
1Department of Medical Biochemistry and Biophysics, Umeå University, Umeå 90187, Sweden. nasim.sabouri@umu.se.
Abstract:
Phen-DC3 is among the most commonly used G-quadruplex (G4)-stabilizers in vitro and in cells. Here, we show that the G4-interactive binding interactions enable one to tune the optical properties of Phen-DC3 allowing the detection of G4 structures in cancer cells. This work opens up new directions for the use of Phen-DC3 as a selective G4 fluorescent reporter.

