Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.1K
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

8.2K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
8.2K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.9K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting Warburg effect in Chinese hamster ovary cell culture with pyruvate dehydrogenase kinase inhibitors.

Biotechnology progress·2026
Same author

Osmolyte-Based Formulations for Enhanced Thermal Stability of mRNA Drug Substance: A Systematic Screening and Optimization Study.

Pharmaceutical research·2026
Same author

Structural Elucidation of Fc- and Fab-Associated <i>N</i>-Glycans in Cetuximab Using Protein A-Assisted Domain-Resolved Glycan Profiling Using Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Bioinformatic pipeline to identify potential therapeutic targets with subsequent isolation and characterization of novel human anti- DDR1 antibodies.

Scientific reports·2026
Same author

Rapid Identification of Counterfeit Biopharmaceuticals using Portable Fourier Transform Infrared Spectroscopy.

AAPS PharmSciTech·2026
Same author

A Multitechnique Spectroscopy Platform for Monitoring Heterogeneities in the Higher-Order Structure of mAb Therapeutics.

Molecular pharmaceutics·2026

Related Experiment Video

Updated: Dec 3, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

44.7K

High-Throughput Process Development: II-Membrane Chromatography.

Anurag S Rathore1, S Muthukumar2

  • 1Department of Chemical Engineering, Indian Institute of Technology, New Delhi, India. asrathore@biotechcmz.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 31, 2020
PubMed
Summary
This summary is machine-generated.

This study presents a protocol for high-throughput process development (HTPD) of membrane chromatography, a faster alternative to traditional methods. It demonstrates reproducible results comparable to lab-scale experiments, valuable for biopharmaceutical development.

Keywords:
AcroPrep™ Advance filter plate with Mustang S membraneDesign of experiments (DOE)Design spaceHigh-throughput process development (HTPD)Ion-exchange chromatography (IEX)Membrane chromatographyMiniaturization

More Related Videos

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.8K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.3K

Related Experiment Videos

Last Updated: Dec 3, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

44.7K
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.8K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.3K

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Analytical Chemistry

Background:

  • Membrane chromatography offers advantages over conventional column chromatography, including reduced pressure drop and faster solute transport.
  • It is increasingly preferred for biopharmaceutical polishing, particularly for monoclonal antibodies, due to time and resource constraints in the industry.

Purpose of the Study:

  • To describe a protocol for high-throughput process development (HTPD) of membrane chromatography.
  • To detail the operation of a specific 96-well format membrane chromatography device (AcroPrep™ Advance filter plate with Mustang S membrane).
  • To address challenges and provide solutions for membrane chromatography experiments and data analysis.

Main Methods:

  • Utilized a commercially available 96-well membrane chromatography device with 7 μL membrane per well.
  • Developed and applied a protocol for HTPD of membrane chromatography steps.
  • Included a case study on ion-exchange chromatography of Granulocyte Colony Stimulating Factor (GCSF).

Main Results:

  • Demonstrated the protocol's ability to generate data representative of traditional lab-scale experiments.
  • Achieved significant agreement between HTPD and lab-scale data, with a regression coefficient of 0.9866.
  • Highlighted the protocol's utility in addressing experimental challenges and facilitating statistical data analysis.

Conclusions:

  • The developed protocol is valuable for high-throughput process development of membrane chromatography.
  • This approach enables efficient and reproducible process development for biopharmaceuticals.
  • The findings support membrane chromatography as a key technology in modern biopharmaceutical manufacturing.