HEAT TREATED BONE ALLOGRAFT AS AN ANTIBIOTIC CARRIER FOR LOCAL APPLICATION

The objective of the study was to assess antibacterial activity of antibiotic-impregnated bone allograft processed according to the Marburg bone bank system. In the experiment, thermal processed bone allografts according to the Marburg bone bank system were used. They were impregnated with gentamicin. Four groups are formed depending on the impregnation method. In I and II groups, an entire femoral heads and, in III and IV perforated femoral heads were used. In groups I and III, antibiotic impregnation was carried out simultaneously with the thermal disinfection, and in groups II and IV after thermal disinfection. In the control group, the bone allografts were heat treated, then impregnated with saline. The antibiotic release was evaluated by agar diffusion test against Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia. The results of the study indicate the presence of antimicrobial activity against S. aureus, P. aeruginosa and K. pneumonia in all groups except the control group. However, in the II and IV group, the inhibition zone was higher compared with the I and III groups (p<0.05). We conclude that the capacity of bone allografts processed according to the Marburg bone bank system to act as gentamicin carriers has been confirmed in this study. Gentamycin loaded perforated bone allograft (IV group) showed statistically higher efficacy against S. aureus, P. aeruginosa and K. pneumonia among studied groups.