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A Coumarin-Based Fluorescent Probe for Ratiometric Detection of Cu2+ and Its Application in Bioimaging
Jie Zhang1, Meng-Yu Chen1, Cui-Bing Bai1,2,3
1School of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, China.
Frontiers in Chemistry
|November 2, 2020
Summary
A new fluorescent probe, L, selectively detects copper ions (Cu2+) with visible color and fluorescence changes. This probe shows potential for sensitive copper detection in various environments, including biological systems.
Area of Science:
- Chemical Sensing
- Materials Science
- Biomedical Applications
Background:
- Copper ions (Cu2+) play crucial roles in biological processes, but their dysregulation is linked to diseases.
- Sensitive and selective detection methods for Cu2+ are essential for research and diagnostics.
- Fluorescent probes offer advantages in sensitivity and real-time monitoring.
Purpose of the Study:
- To design, synthesize, and characterize a novel fluorescent probe (L) for selective Cu2+ recognition.
- To evaluate the probe's performance in various conditions, including aqueous and biological samples.
- To investigate the sensing mechanism and potential applications in bioimaging.
Main Methods:
- Synthesis and characterization of the naphthalimide-coumarin based fluorescent probe L.
- Spectroscopic studies (UV-Vis, fluorescence) to assess selectivity and sensitivity towards Cu2+.
- Job's plot, IR, MS, SEM, and 1HNMR for recognition mechanism elucidation.
- Density Functional Theory (DFT) calculations for mechanistic insights.
- Application testing in tap water and Caenorhabditis elegans.
Main Results:
- Probe L exhibited selective and sensitive recognition of Cu2+ in HEPES buffer/CH3CN.
- Interaction with Cu2+ caused distinct color and fluorescence intensity changes, enabling naked-eye detection.
- The probe demonstrated effective Cu2+ detection in tap water using test strips.
- A low limit of detection (LOD) of 3.5 × 10-6 M was achieved for Cu2+.
- Probe L showed favorable selectivity for Cu2+ in Caenorhabditis elegans, indicating bioimaging potential.
Conclusions:
- The developed fluorescent probe L is highly selective and sensitive for Cu2+ detection.
- Probe L offers a simple and effective method for Cu2+ sensing, applicable in diverse environments.
- The probe's performance in biological models suggests its potential utility in bioimaging and diagnostics.
