Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Loss of ASIC1A-dependent inhibitory neuron activity in basolateral amygdala is associated with increased CO <sub>2</sub> -evoked jumping.

bioRxiv : the preprint server for biology·2026

Same author

A DNA Methylation-based algorithm Improves Lung Cancer risk prediction in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial.

Lung cancer (Amsterdam, Netherlands)·2026

Same author

Global functional connectivity of cognitive control networks predicts task-switching performance in older adults.

Cortex; a journal devoted to the study of the nervous system and behavior·2026

Same author

Methylation Biomarker of Chronic Heavy Alcohol Consumption (HAC), but Not Acute HAC, Predicts All-Cause Mortality in Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial.

Genes·2026

Same author

Nonexercise Equations for Cardiorespiratory Fitness in Older Adults using Body Roundness Index and Waist Circumference.

Exercise, sport & movement·2025

Same author

An Illustration of the Protective Value of Epigenetics: Using the Alcohol T Score (ATS) in A Population of Known Smokers.

Journal of insurance medicine (New York, N.Y.)·2025

Same journal

Epigenetic regulation of vertebrate limb development: from field specification to tissue morphogenesis.

Epigenetics·2026

Same journal

Dnmt1 associated <i>Gja1</i> promoter methylation changes are implicated in Cx43 remodeling during acute myocardial ischemia/reperfusion injury.

Epigenetics·2026

Same journal

Differential CpG methylation in the <i>Interferon Gamma</i> (<i>IFNG</i>) promoter correlates with viral load and transcriptional control in people with HIV.

Epigenetics·2026

Same journal

m6A methylation of circKPNA2 promotes colorectal carcinogenesis by activating the RIN1-Ras pathway.

Epigenetics·2026

Same journal

The epigenetic archaeology of human-dog companionship.

Epigenetics·2026

Same journal

LncRNA MIR4435-2HG-mediated succinylation of USF1 promotes its protein stability and induces epithelial-mesenchymal transition in HNSCC.

Epigenetics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 2, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

A simple, rapid, interpretable, actionable and implementable digital PCR based mortality index.

Robert Philibert^1,2, Jeffrey D Long^1,3, James A Mills¹

¹Department of Psychiatry, University of Iowa, Iowa City, IA, USA.

|November 3, 2020

Summary

This summary is machine-generated.

This study introduces a novel five-locus DNA methylation algorithm for mortality risk assessment. This epigenetics tool is clinically translatable, free of racial bias, and outperforms existing indices.

Keywords:

DNA methylation alcohol coronary artery disease diabetes mortality smoking

More Related Videos

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Related Experiment Videos

Last Updated: Dec 2, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Area of Science:

Epigenetics
Biomarkers
Mortality Risk Assessment

Background:

DNA methylation tools are advancing mortality risk assessment for civil and commercial use.
Current array-based algorithms lack clinical translatability and may exhibit racial bias.
Need for reliable, unbiased epigenetic biomarkers for clinical application.

Purpose of the Study:

Develop and validate a novel, clinically translatable mortality-risk algorithm using DNA methylation.
Ensure the algorithm is free from racial bias and assesses key health factors.
Compare the algorithm's performance against existing epigenetic clocks.

Main Methods:

Utilized Framingham Heart Study (FHS) array data to develop a five-locus methylation algorithm.
Selected validated methylation biomarkers for smoking, alcohol, diabetes, and heart disease.
Validated loci using digital PCR and assessed for ethnic variation.

Main Results:

The five-locus algorithm, incorporating age and sex, outperformed the Levine index and matched GrimAge's predictive power.
Digital PCR confirmed the clinical translatability of the selected loci.
The algorithm's methylation loci did not show ethnically specific variation.

Conclusions:

A simple, powerful, and clinically translatable five-locus DNA methylation algorithm for mortality risk assessment has been developed.
This tool can provide patient feedback, guide medical assessments, and monitor public health interventions.
The algorithm offers an unbiased and effective approach to mortality risk prediction.