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Updated: Dec 2, 2025
14:51
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Published on: November 13, 2021
5.8K
Spatially resolved absolute quantitation in thin tissue by mass spectrometry.
Vilmos Kertesz1, John F Cahill2
1Oak Ridge National Laboratory, Oak Ridge, TN, 37831-6131, USA. kerteszv@ornl.gov.
Analytical and Bioanalytical Chemistry
|November 3, 2020
Summary
Quantitative mass spectrometry (MS) imaging and profiling of tissues is crucial for clinical and pharmacological applications. This review covers novel MS technologies for spatial analysis, addressing biases to improve data accuracy.
Area of Science:
- Biomedical analysis
- Analytical chemistry
- Molecular imaging
Background:
- Mass spectrometry (MS) is a primary tool for biomolecule quantification.
- Spatially resolved MS technologies are emerging for analyzing biomolecule distribution in tissues.
- Current spatially resolved MS methods often yield qualitative data due to inherent biases.
Purpose of the Study:
- To review MS profiling and imaging technologies capable of quantitative analysis from tissue.
- To discuss fundamental processes that introduce biases in quantitative MS.
- To present methods for addressing these biases in spatially resolved MS.
Main Methods:
- Overview of various MS imaging and profiling technologies.
- Analysis of factors affecting quantitative accuracy in MS.
- Discussion of bias mitigation strategies.
Main Results:
- Several MS technologies offer varying degrees of spatial and quantitative information.
- Understanding and addressing biases are critical for accurate quantitative MS.
- Methods exist to improve the reliability of quantitative MS data from tissues.
Conclusions:
- Quantitative spatially resolved MS is essential for advancing pharmacological and clinical research.
- Continued development of MS technologies and bias correction methods is needed.
- This review provides a foundation for selecting and applying quantitative MS techniques.
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