Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

705
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
705
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.4K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.4K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

817
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
817
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

3.3K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
3.3K
Chromatography: Introduction01:10

Chromatography: Introduction

6.3K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
6.3K
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

3.1K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatocyte purification column using thermoresponsive glycopolymer-modified silica beads.

Journal of materials chemistry. B·2026
Same author

Optimization of the composition of temperature-responsive polymers for spin columns.

Scientific reports·2026
Same author

Deproteination of Serum-Drug Samples Utilizing a Temperature-Responsive Spin Column Containing Thermoresponsive Polymer Brush-Modified Beads.

Biological & pharmaceutical bulletin·2025
Same author

Thermo-responsive targeting of polymeric micelles by controlling the cellular uptake based on the change of their surface arginine density.

Communications chemistry·2025
Same author

Therapeutic Drug Monitoring Using Two-Dimensional Chromatography System Composed of Two Types of Temperature-Responsive Chromatography Columns.

Chemical & pharmaceutical bulletin·2025
Same author

Therapeutic Drug Monitoring Using a Poly(N-isopropylacrylamide) Hydrogel-Modified Bead-Packed Column with an All-Aqueous Mobile Phase without Sample Deproteinization.

Chemical & pharmaceutical bulletin·2025
Same journal

Programmable DNA probe-mediated nanopore biosensor for multiplex nucleic acid detection and its application in milk authenticity identification.

Analytica chimica acta·2026
Same journal

A multifunctional fluorescent sensor for sequential off-on-off visual detection of Zn<sup>2+</sup> and glyphosate in food and biological matrices and efficient removal of Zn<sup>2+</sup> from aqueous media.

Analytica chimica acta·2026
Same journal

Automated carousel-based electrochemical sensing toward microbiological and oncological settings.

Analytica chimica acta·2026
Same journal

Label-free quantification of cumulative cytosol-enriched peptide concentrations by mass spectrometry.

Analytica chimica acta·2026
Same journal

Integrated multi-matrix bile acid metabolic metrics (BAMMs): A methodological framework for functional metabolic phenotyping in human subjects.

Analytica chimica acta·2026
Same journal

A dual-enzymatic activity/SERS dual-mode sensor array based on BSA-Cu nanoflowers for sensitive detection of various foodborne pathogens.

Analytica chimica acta·2026
See all related articles

Related Experiment Video

Updated: Dec 1, 2025

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

68.9K

Temperature-responsive chromatography for bioseparations: A review.

Kenichi Nagase1, Hideko Kanazawa1

  • 1Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.

Analytica Chimica Acta
|November 9, 2020
PubMed
Summary
This summary is machine-generated.

Temperature-responsive chromatography using poly(N-isopropylacrylamide) enables effective bioseparation. This advanced technique separates diverse analytes like drugs, proteins, and cells without compromising their activity.

Keywords:
BiopharmaceuticalsBioseparationCell separationTemperature-responsive chromatographyThermoresponsive polymers

More Related Videos

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

44.7K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.3K

Related Experiment Videos

Last Updated: Dec 1, 2025

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

68.9K
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

44.7K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.3K

Area of Science:

  • Biomedical technology and separation science.
  • Polymer chemistry and chromatography.

Background:

  • Biopharmaceutical and cell-based therapies require advanced separation techniques.
  • Existing methods face challenges in preserving the integrity of biological materials.
  • Poly(N-isopropylacrylamide) (PNIPAM) based systems offer temperature-responsive separation.

Purpose of the Study:

  • To review the development and application of temperature-responsive chromatography for bioseparation.
  • To highlight the versatility of PNIPAM-based systems in separating diverse biomolecules and cells.
  • To assess the potential of these methods for future bioseparation challenges.

Main Methods:

  • Development of temperature-responsive chromatography systems utilizing poly(N-isopropylacrylamide) and its copolymers.
  • Modification of copolymer composition, polymer graft configuration, and stationary phase materials.
  • Application of temperature changes to control separation processes in chromatography columns.

Main Results:

  • Successful separation of small-molecule drugs, peptides, and proteins using temperature-responsive chromatography.
  • Demonstrated preservation of biological activity during the separation of sensitive biomolecules.
  • Investigated temperature-modulated cell separation columns for cell isolation without property alteration.

Conclusions:

  • Temperature-responsive chromatography offers a versatile and effective platform for bioseparation.
  • These methods are crucial for the analysis and production of biopharmaceuticals and therapeutic cells.
  • The technology holds significant promise for current and future applications in biotechnology and medicine.