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Updated: Dec 1, 2025

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Physiological and Performance Impacts After Field Supramaximal High-Intensity Interval Training With Different
Leandro Henrique Albuquerque Brandão1,2, Thaysa Passos Nery Chagas3, Alan Bruno Silva Vasconcelos2,3
1Post-Graduate Program of Physical Education, Federal University of Sergipe, São Cristovão, Brazil.
High-intensity interval training (HIIT) protocols with different work-recovery durations (15:15 vs. 30:30) elicited similar physiological responses and performance in recreational runners. The 30:30 protocol showed slightly lower post-exercise creatine kinase and lactate levels.
Area of Science:
- Exercise Physiology
- Sports Science
- Biochemistry
Background:
- High-intensity interval training (HIIT) involves adjustable external load variables that influence athlete responses.
- Understanding the impact of different HIIT work-to-rest ratios on physiological markers is crucial for training optimization.
Purpose of the Study:
- To compare the effects of two distinct HIIT protocols (15:15 and 30:30 work-recovery) on enzyme activity (CK, LDH), performance, and metabolic responses in male recreational runners.
- To analyze blood lactate and glucose levels, and creatine kinase and lactate dehydrogenase enzyme activities following the HIIT protocols.
Main Methods:
- Fifteen male recreational runners participated in two randomized HIIT protocols (15:15 and 30:30) at 130% vV̇O2max until exhaustion.
- Blood samples were collected pre-exercise and at 5 and 10 minutes post-exercise for lactate and glucose measurements.
- Creatine kinase (CK) and lactate dehydrogenase (LDH) enzyme activities were measured 24 hours before and 48 hours after each protocol.
Main Results:
- No significant differences in overall performance (distance, time) or blood parameters were observed between the 15:15 and 30:30 HIIT protocols.
- Both protocols induced significant changes in blood lactate, CK, and LDH levels over time.
- The 30:30 protocol demonstrated a trend towards lower CK activity at 48 hours post-exercise and reduced lactate levels at 10 minutes post-exercise compared to the 15:15 protocol.
Conclusions:
- HIIT protocols with 15:15 and 30:30 work-recovery ratios produce comparable physiological and performance outcomes in recreational male runners.
- While overall responses are similar, the 30:30 protocol may offer a slightly reduced muscle damage and metabolic stress profile.
- Training prescription should consider individual athlete responses and recovery when selecting HIIT work-to-rest ratios.
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