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Updated: Dec 1, 2025
Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Development and validation of an improved 3-methyl-2-benzothiazolinone hydrazone method for quantitative
Xingshuang Lv1, Pengbo Wang1, Tengbin Wang1
1College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Abstract:
An accurate and sensitive analytical method for detecting and quantifying reducing sugar ends (RSE) in chitooligosaccharides (COSs) is the key quality parameter for evaluating their structure-function relationship and potential applications. In this work, we develop and validate a novel colorimetric assay with high accuracy and precision for determining RSE content using 3-methyl-2-benzothiazolinone hydrazone (MBTH). Under optimal conditions, the stoichiometry is verified using mono-, di-, and tri- glucosamine hydrochlorides, and the dilution ratio does not interfere with the RSE content measured at 590 nm. The regression equation of glucosamine reveal a good linear relationship (R2 = 0.9999). The detection limit, quantification limit, mean relative standard deviation (RSD), and recovery are 2.28 μM, 9.11 μM, 1.90%, and 98.0%, respectively. The newly developed method is potentially useful for monitoring COS hydrolysis, number average molecular weight, and chitosanase activity.
