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Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces
Wei Sun1,2,3, Dandan Ma3, Jan G M Bolscher3
1The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Frontiers in Bioengineering and Biotechnology
|November 16, 2020
Summary
Human salivary histatin-1 (Hst-1) significantly enhances osteogenic cell spreading, a key factor for bone healing and implant integration. This effect involves extracellular-signal-regulated kinase (ERK) and p38 signaling pathways.
Area of Science:
- Biomaterials Science
- Cell Biology
- Regenerative Medicine
Background:
- Cell spreading is critical for successful bone regeneration and osseointegration of medical implants.
- Understanding the molecular mechanisms that promote cell spreading is essential for developing new therapeutic strategies.
Purpose of the Study:
- To investigate the effect of human salivary histatin-1 (Hst-1) on osteogenic cell spreading in vitro.
- To elucidate the signaling pathways involved in Hst-1-mediated cell spreading.
Main Methods:
- Osteogenic cells were cultured on various substrates with or without Hst-1.
- Cell spreading was quantified using a point-counting method.
- Signaling pathways were assessed using specific inhibitors (U0126, SB203580, PTx) and fluorescent microscopy.
Main Results:
- Hst-1 significantly promoted osteogenic cell spreading at an optimal concentration of 10 μM.
- Truncated or scrambled Hst-1 variants did not show a similar effect.
- Hst-1-induced spreading was dependent on extracellular-signal-regulated kinase (ERK) and p38 pathways, but not G protein-coupled receptors.
Conclusions:
- Human salivary histatin-1 is a potent stimulator of osteogenic cell spreading.
- Hst-1 holds promise as a therapeutic peptide to improve bone healing and dental implant osseointegration.
