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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
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Acoustic Ejection/Full-Scan Mass Spectrometry Analysis for High-Throughput Compound Quality Control.

Jun Zhang1, Yong Zhang1, Chang Liu2

  • 1Research and Early Development, Bristol Myers Squibb, Princeton, NJ, USA.

SLAS Technology
|November 16, 2020
PubMed
Summary

Acoustic Ejection-Mass Spectrometry (AE-MS) offers a rapid, low-consumption method for high-throughput compound quality control (QC). This technique significantly reduces analysis time and sample usage compared to traditional LC-MS, streamlining drug discovery workflows.

Keywords:
DMSO sampleacoustic ejectioncompound QChigh-throughputmass spectrometry

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Drug Discovery

Background:

  • High-throughput compound quality control (QC) is crucial in drug discovery for assay-ready plates, compound integrity, and reaction monitoring.
  • Traditional liquid chromatography-mass spectrometry (LC-MS) workflows face significant throughput limitations due to the large number of samples involved.
  • Existing chromatography-free mass spectrometry (MS) approaches have yielded mixed results for rapid analysis.

Purpose of the Study:

  • To demonstrate the feasibility of acoustic ejection-mass spectrometry (AE-MS) for high-throughput compound QC in miniaturized formats.
  • To evaluate AE-MS for both qualitative and quantitative analyses, aiming for reduced sample consumption and increased analytical speed.

Main Methods:

  • Utilized acoustic ejection-mass spectrometry (AE-MS) in full-scan mode for direct, contactless liquid sampling.
  • Compared AE-MS performance against conventional LC-MS for qualitative QC and relative quantification.
  • Developed a data processing platform using commercial tools to manage AE-MS generated data.

Main Results:

  • AE-MS achieved a 1000-fold reduction in sample consumption and a 30-fold reduction in analysis time compared to LC-MS.
  • AE-MS provided comparable results to LC-MS for identifying the presence/absence of compounds in qualitative QC.
  • AE-MS demonstrated utility in the relative quantification of compounds in serial dilutions and reaction substrates.

Conclusions:

  • Acoustic Ejection-Mass Spectrometry (AE-MS) is a viable and efficient technique for high-throughput compound QC.
  • AE-MS significantly overcomes the analytical bottleneck in compound QC workflows, offering substantial improvements in speed and sample economy.
  • The developed data processing platform facilitates straightforward data handling, eliminating the need for custom software.