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Published on: March 17, 2016
Identification of human parvovirus 4 genotypes 1 and 2 in Chinese source plasma pools
Junting Jia1,2, Yadi Zhong2,3, Huan Zhang2
1Department of Transfusion Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing, China.
Abstract:
Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) are known to infect humans and transmit through contaminated blood and blood products. Globally, three genotypes of B19V, as well as PARV4, have been identified, respectively. The existence of different B19V genotypes in Chinese plasma donors has been investigated, however, the data regarding PARV4 were not available. The main objective of this study is to identify the genotypes of PARV4 circulating in Chinese plasma donors. By using a duplex quantitative polymerase chain reaction assay adapted for all genotypes of B19V and PARV4, 78 source plasma pools for fractionation were screened and quantified. Results showed that positive rates of B19V and PARV4 DNA in plasma pool samples were 25.64% and 14.10%, respectively. PARV4 sequences in two positive samples were next genotyped, and these two sequences belonged to PARV4 genotypes 1 and 2, respectively. In conclusion, the data present demonstrate the existence of PARV4 genotypes 1 and 2 in Chinese plasma donors for the first time and also show the relatively lower prevalence and level of PARV4 DNA in Chinese plasma donors in comparison with that of B19V DNA.
Insights
Human Parvovirus 4 (PARV4) genotypes 1 and 2 were identified in Chinese plasma donors for the first time. This study found PARV4 DNA at lower levels than Human Parvovirus B19 (B19V) DNA.
Area of Science:
- Virology
- Molecular Biology
- Public Health
Background:
- Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) are known human pathogens transmitted via blood products.
- While B19V genotypes are documented in China, PARV4 data in Chinese plasma donors were lacking.
Purpose of the Study:
- To identify circulating PARV4 genotypes in Chinese plasma donors.
- To compare the prevalence of PARV4 with B19V in this population.
Main Methods:
- A duplex quantitative polymerase chain reaction (qPCR) assay was developed and validated for B19V and PARV4.
- 78 source plasma pools were screened and quantified using the developed qPCR assay.
Main Results:
- Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) DNA were detected in 25.64% and 14.10% of plasma pools, respectively.
- Genotyping revealed the presence of PARV4 genotypes 1 and 2 in positive samples.
- PARV4 DNA showed lower prevalence and concentration compared to B19V DNA.
Conclusions:
- This study reports for the first time the presence of PARV4 genotypes 1 and 2 in Chinese plasma donors.
- PARV4 appears less prevalent and at lower levels than B19V in this donor population.
