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Universal Platform for Ratiometric Sensing Based on Catalytically Induced Inner-Filter Effect by Cu2.

Yongchao Fan1,2, Huanhuan Xing1,2, Yuan Xue1,2

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.

Analytical Chemistry
|November 19, 2020
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Summary

A novel ratiometric fluorescent sensor accurately detects Cu2+ by converting o-phenylenediamine into 2,3-diaminophenazine. This method also enables sensitive detection of glutathione (GSH), offering a new route for analyzing small biological molecules.

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Biomolecular Detection

Background:

  • Accurate assays require integrating multiple fluorescent probes into a single sensing platform.
  • Ratiometric sensing offers enhanced accuracy and sensitivity compared to single-probe methods.
  • Developing enzyme-free platforms for biological molecule detection is crucial.

Purpose of the Study:

  • To develop a sensitive ratiometric fluorescent sensor for Cu2+ detection.
  • To utilize the inner-filter effect for improved sensing performance.
  • To establish a platform for detecting glutathione (GSH) based on Cu2+ interaction.

Main Methods:

  • In situ synthesis of 2,3-diaminophenazine (DAP) from o-phenylenediamine (OPD) catalyzed by Cu2+.
  • Utilizing the overlapped spectra of 2-aminoterephthalic acid (PTA-NH2) and DAP for ratiometric detection.
  • Employing a dual-emission reverse change ratiometric profile based on the inner-filter effect.

Main Results:

  • A highly sensitive ratiometric fluorescent sensor for Cu2+ was developed with a detection limit of 1.7 nmol·L-1.
  • The sensor demonstrated a wide detection range for Cu2+ (5-200 nmol·L-1).
  • The platform showed high sensitivity for glutathione (GSH) detection (0.5-80 μmol·L-1, LOD 0.16 μmol·L-1).

Conclusions:

  • The developed ratiometric sensing platform provides a simple and effective method for Cu2+ detection.
  • The sensor exhibits high selectivity and sensitivity for both Cu2+ and GSH.
  • This enzyme-free approach offers a new avenue for detecting biologically relevant small molecules.