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CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells
Mateusz Kotowski1, Sumana Sharma1
1MRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UK.
Methods and Protocols
|November 21, 2020
Summary
Genome editing with clustered regularly interspaced short palindromic repeats (CRISPR) is now precise and scalable in primary T cells. This advance promises to deepen understanding of T cell biology and revolutionize T cell therapies.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Genome editing in immortalized immune cells is established.
- Efficient genetic manipulation of primary T cells remains challenging.
Purpose of the Study:
- To review primary T cell editing techniques.
- To discuss advancements in CRISPR-based tools for T cell engineering.
Main Methods:
- Review of CRISPR-based genome editing techniques.
- Analysis of delivery methods for CRISPR components into primary T cells.
Main Results:
- CRISPR toolbox expansion enables precise and large-scale genetic manipulation of primary T cells.
- New generation CRISPR tools offer enhanced capabilities for T cell engineering.
Conclusions:
- Improved primary T cell editing advances fundamental understanding of T cell signaling and transcriptional networks.
- Enhanced genetic manipulation of T cells has the potential to revolutionize T cell-based therapies.
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