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Flexible enzyme cascade sensing platform based on a G-quadruplex nanofiber biohydrogel for target colorimetric
Yanmei Guo1, Huiqi Wang1, Feifei Zhang1
1College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
Abstract:
Enzyme cascade reactions can greatly improve catalytic efficiency and achieve selective and sensitive detection of targets. This paper presents a novel strategy for colorimetric sensing of targets by loading natural enzymes into a hemin-doped G-quadruplex (G4-hemin) biohydrogel to form a flexible enzyme cascade sensing (FECS) platform. The biohydrogel has advantages of biocompatibility, printability and flexibility. The biohydrogel not only participates in the cascade reaction as the mimic enzyme but also provides a mild microenvironment for the natural enzyme. The FECS platform has a linear range from 0.4 μM to 120 μM and a detection limit of 3.6 × 10-6 M for hydrogen peroxide detection. Additionally, the FECS platform has a low detection limit and wide linear range for glucose and xanthine by loading xanthine oxidase (XOD) and glucose oxidase (GOD) into the biohydrogel, respectively. These results indicate that the novel FECS platform is an effective detection platform that can detect multiple targets and is expected to be widely used in flexible sensing.
