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Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
Published on: June 21, 2012
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Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance
Jennifer L Brehm1, Molly M Miles1, Katie S Remondini1
1WiCell Research Institute, Madison, WI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2020
Abstract:
Mouse embryonic fibroblasts (MEFs) can be used in co-culture to support generation of induced pluripotent stem cells (iPSCs) and the normal growth and proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.
