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Microgavage of Zebrafish Larvae
Published on: February 20, 2013
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Probiotics Modulate Intestinal Motility and Inflammation in Zebrafish Models.
Tao Wang1, Ming-Zhu Dai2, Feng-Song Liu1
1Infinitus (China) Company Ltd., Guangzhou, China.
Zebrafish
|November 24, 2020
Summary
Three probiotic strains, including Lactobacillus acidophilus NCFM, improved zebrafish intestinal motility. Lactobacillus rhamnosus HN001 and Bifidobacterium animalis lactis Bi-07 also reduced intestinal inflammation and aided recovery.
Area of Science:
- Microbiology and Immunology
- Zebrafish models in biomedical research
- Gastrointestinal health and probiotics
Background:
- Probiotics are increasingly recognized for their potential to modulate gut health.
- Zebrafish larvae offer a rapid and cost-effective model for studying gastrointestinal physiology and disease.
- Assessing the efficacy of specific probiotic strains requires robust in vivo models.
Purpose of the Study:
- To evaluate the effects of three specific probiotic strains on intestinal motility and inflammation in zebrafish.
- To identify which probiotic strains demonstrate the most significant impact on gut function and health markers.
- To explore the potential of using zebrafish models for accelerated probiotic research and development.
Main Methods:
- Zebrafish models were used to assess intestinal motility via fluorescent dye clearance and image analysis.
- Intestinal inflammation was induced using trinitrobenzenesulfonic acid in fluorescent neutrophil zebrafish.
- Probiotics (Lactobacillus acidophilus NCFM, Lactobacillus rhamnosus HN001, Bifidobacterium animalis lactis Bi-07) were administered at varying concentrations.
Main Results:
- All tested probiotic strains enhanced intestinal movement in zebrafish.
- Bifidobacterium animalis subsp. lactis Bi-07 showed the strongest effect on motility at lower concentrations.
- Lactobacillus rhamnosus HN001 and Bifidobacterium animalis subsp. lactis Bi-07 demonstrated therapeutic effects on intestinal inflammation and mucosal damage.
- L. rhamnosus HN001's anti-inflammatory action involved reducing IL-6 and increasing TGFβ-1; B. animalis subsp. lactis Bi-07's involved TGFβ-1 elevation.
Conclusions:
- Larval zebrafish serve as an effective model for screening probiotics and assessing their impact on gut health.
- Specific probiotic strains, notably L. rhamnosus HN001 and B. animalis subsp. lactis Bi-07, possess significant anti-inflammatory and gut-healing properties.
- This approach can accelerate the development and improve the quality of probiotic products for human health.
