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Related Experiment Videos

Rheumatoid factor.

P P Chen1, S Fong, D A Carson

  • 1Department of Basic and Clinical Research, Research Institute of Scripps, La Jolla, California.

Rheumatic Diseases Clinics of North America
|December 1, 1987
PubMed
Summary
This summary is machine-generated.

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Human rheumatoid factors (RFs) utilize a limited set of immunoglobulin variable region genes, with one specific Vk gene (Humkv325) being common and conserved in diverse populations.

Area of Science:

  • Immunology
  • Molecular Genetics
  • Autoimmunity

Background:

  • Crossreactive idiotypes (CRIs) suggest human rheumatoid factors (RFs) use common immunoglobulin (Ig) variable region genes.
  • RF production can be triggered by various stimuli, including aggregated IgG, exogenous antigens, and polyclonal activators.

Purpose of the Study:

  • To investigate the immunoglobulin variable region gene usage in human RFs.
  • To identify specific genes contributing to RF production and their prevalence in human populations.

Main Methods:

  • Combined protein and DNA sequencing of human RFs.
  • mRNA sequencing for comparison with murine RFs.
  • Cloning and characterization of human RF-related light chain variable region genes.

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Main Results:

  • Human RFs utilize a restricted set of light and heavy chain variable region genes, unlike murine RFs which use diverse genes.
  • A specific germ-line Vk gene, Humkv325, is identical in four unrelated individuals' RFs and differs slightly in others.
  • The Humkv325 gene demonstrates wide distribution and conservation across outbred human populations.

Conclusions:

  • Human RFs are generated from a limited repertoire of Ig variable region genes.
  • The conserved Humkv325 Vk gene is a significant contributor to human RFs, indicating its importance in autoimmunity.