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Structural Organization and Protein-Protein Interactions in Human Adenovirus Capsid

Vijay S Reddy1, Michael A Barry2

  • 1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. reddyv@scripps.edu.

Sub-Cellular Biochemistry
|November 30, 2020
PubMed

Insights

Human adenoviruses (HAdVs) are complex viruses causing infections and used in therapies. Advanced cryo-electron microscopy reveals their structural organization and protein interactions for improved vector design.

Area of Science:

  • Virology
  • Structural Biology
  • Biochemistry

Background:

  • Human adenoviruses (HAdVs) are nonenveloped dsDNA viruses responsible for various infections.
  • HAdVs pose health risks, particularly to vulnerable populations.
  • Adenoviruses are crucial as vectors in gene, vaccine, and cancer therapies.

Purpose of the Study:

  • To detail the current understanding of human adenovirus structure and organization.
  • To explore the protein-protein interactions within the adenovirus capsid.
  • To highlight how structural insights inform the design of novel adenovirus vectors.

Main Methods:

  • Utilizing advancements in cryo-electron microscopy (cryo-EM) for 3D structure determination.
  • Analyzing near-atomic resolution structures of adenovirus architecture.
  • Investigating the role of specific proteins, like cement/minor proteins, in capsid stabilization.

Main Results:

  • Cryo-EM has unveiled the intricate organization of adenovirus architecture.
  • Identified the network of protein-protein interactions stabilizing the viral capsid.
  • Observed conserved structural features and protein interactions among human adenoviruses.

Conclusions:

  • The detailed structure of human adenoviruses is increasingly understood through cryo-EM.
  • Understanding capsid protein interactions is key to adenovirus vector development.
  • Structural insights facilitate the design of modified adenoviruses with enhanced functionalities.
Keywords:
Adenovirus structureCement proteinsHexonPenton baseProtein IIIaProtein IXProtein VIII and protein VIProtein networkProtein-protein interactions

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