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Dynamic transcriptome response in Meretrix meretrix to Aroclor 1254 exposure
Yongqi Li1, Shishi Liu1, Hanliang Cheng1
1Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China.
Polychlorinated biphenyls (PCBs) disrupt clam metabolism, affecting immune and endocrine systems. Transcriptome analysis revealed significant gene expression changes in response to Aroclor 1254 exposure, indicating toxic effects.
Area of Science:
- Environmental Toxicology
- Marine Biology
- Molecular Biology
Background:
- Polychlorinated biphenyls (PCBs) are persistent organic pollutants with known toxic effects on various biological systems.
- Understanding the molecular mechanisms of PCB toxicity in marine organisms is crucial for assessing environmental risks.
Purpose of the Study:
- To investigate the molecular effects of Aroclor 1254 exposure on the transcriptome of the clam Meretrix meretrix.
- To identify differentially expressed genes involved in detoxification, oxidative stress, immune response, and endocrine disruption.
Main Methods:
- Clams (Meretrix meretrix) were exposed to Aroclor 1254 (10 and 1000 ng/L) and natural seawater (control).
- Transcriptome analysis was performed on samples collected after 1 and 3 days of exposure.
- Differential gene expression analysis was conducted to identify significant changes.
Main Results:
- Over 535,000 transcripts were assembled, resulting in 177,142 unigenes.
- 5101 differentially expressed genes were identified, primarily related to detoxification, oxidative stress, immune response, and endocrine disruption.
- Specific genes like cytochrome P450s, glutathione S-transferase, lysozyme, and vitellogenin showed significant induction or repression, with some exhibiting time and dose-dependent responses.
Conclusions:
- Aroclor 1254 exposure significantly alters gene expression in Meretrix meretrix, impacting key physiological processes.
- The identified genes provide insights into the molecular pathways affected by PCB toxicity in marine bivalves.
- This study contributes to understanding the ecotoxicological impact of PCBs on marine ecosystems.
