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Related Experiment Video

Updated: Nov 27, 2025

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

Lotus-Root-Like Microchanneled Collagen Scaffold.

Hanjun Hwangbo¹, WonJin Kim¹, Geun Hyung Kim^1,2

¹Department of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea.

ACS Applied Materials & Interfaces

|December 2, 2020

Summary

This summary is machine-generated.

Researchers developed a novel biofabrication method to create aligned microtubular collagen scaffolds. These lotus-like structures effectively guide cell alignment and promote muscle tissue regeneration, offering a promising approach for tissue engineering.

Keywords:

3D printing biomimetic collagen lotus-root-like scaffold skeletal muscle

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Last Updated: Nov 27, 2025

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

Interlinked Macroporous 3D Scaffolds from Microgel Rods

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Published on: June 16, 2022

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

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Published on: January 7, 2019

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Microtubular tissue structures like muscles and vessels are vital in the human body.
Tissue engineering scaffolds offer aligned niches to guide cell recruitment and differentiation for tissue regeneration.
Extracellular matrix (ECM)-derived scaffolds provide excellent biocompatibility, but their fabrication into complex microtubular structures is challenging.

Purpose of the Study:

To develop an innovative biofabrication system for creating uniaxially aligned microtubular collagen scaffolds.
To investigate the influence of material and processing factors on scaffold morphology.
To evaluate the physical and biological activities of the fabricated scaffolds compared to controls.

Main Methods:

A sequential removal of supporting materials (polycaprolactone (PCL) and poly(vinyl alcohol) (PVA)) was employed.
Collagen coating weight fraction and PVA molecular weight were manipulated to achieve specific lotus-like structures.
Physical and biological assessments were performed on aligned hierarchical microtubular collagen scaffolds, conventional collagen struts, and non-aligned microtubular scaffolds.

Main Results:

A novel biofabrication system successfully produced uniaxially aligned microtubular collagen scaffolds with a lotus-like structure.
The aligned hierarchical microtubular collagen structure demonstrated superior physical and biological properties compared to controls.
Significant myoblast alignment and enhanced myogenic differentiation were observed on the aligned scaffolds.

Conclusions:

The developed biofabrication approach enables the creation of instructive, aligned microtubular collagen scaffolds.
The unique topographical cues of the aligned hierarchical microtubular structure are crucial for guiding cell behavior.
These scaffolds show high potential for applications in muscle tissue regeneration.