Droplet Microarray Based on Nanosensing Probe Patterns for Simultaneous Detection of Multiple HIV Retroviral Nucleic...

Area of Science:

• Biotechnology
• Materials Science
• Molecular Diagnostics

Background:

• Multiplexed detection of viral nucleic acids is crucial for efficient infectious disease screening.
• Existing methods may lack sensitivity or the ability to detect multiple viruses simultaneously.

Purpose of the Study:

• To develop a sensitive and rapid multiplexed detection platform for retroviral nucleic acids.
• To demonstrate simultaneous detection of human immunodeficiency virus-1 (HIV-1) and human immunodeficiency virus-2 (HIV-2).

Main Methods:

• Fabrication of a dendrimer droplet microarray (DMA) using omniphobic-omniphilic patterning.
• Modification of MoS2 nanosheets with fluorescently labeled oligomer probes for FRET sensing.
• Utilizing microdroplet formation and target gene hybridization to trigger fluorescence signal recovery.

Main Results:

• Achieved simultaneous detection of HIV-1 and HIV-2 nucleic acids with a limit of detection (LOD) of 50 pM.
• Demonstrated multiplexed detection of five viral genes, including SARS-CoV-2 and Influenza A.
• The platform requires a small sample volume (<150 nL).

Conclusions:

• The MoS2-DMA FRET sensing platform shows significant potential for high-throughput multiplexed viral nucleic acid screening.
• This technology enables rapid and sensitive detection of multiple viral pathogens.
• The developed microarray offers a promising tool for infectious disease diagnostics.