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Linear flow-velocity gradient chromatography-An efficient method for increasing the process efficiency of batch and
Chyi-Shin Chen1, Kosei Ando1, Noriko Yoshimoto1,2
1Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube, Japan.
Biotechnology and Bioengineering
|December 7, 2020
Summary
A novel linear flow-velocity gradient (LFG) method enhances capture chromatography efficiency. This technique significantly boosts productivity and reduces costs in processing monoclonal antibodies (mAbs).
Area of Science:
- Biotechnology
- Chemical Engineering
- Chromatography
Background:
- Capture chromatography is crucial for biopharmaceutical purification.
- Optimizing process efficiency, such as flow velocity, is key to reducing costs and increasing throughput.
- Current methods often use constant flow velocity, which may not be optimal for all conditions.
Purpose of the Study:
- To introduce and validate a new linear flow-velocity gradient (LFG) method for enhancing capture chromatography efficiency.
- To develop a systematic approach for determining the optimal parameters for LFG operation.
- To assess the impact of LFG on productivity and cost-effectiveness in monoclonal antibody (mAbs) purification.
Main Methods:
- Proposed a linear decreasing flow-velocity gradient (LFG) method during sample loading.
- Developed a parameter determination method using total column capacity and loaded amount as a function of time.
- Calculated capacity using dynamic binding capacity (DBC) and residence time relationships.
- Verified the method using ion-exchange and Protein A chromatography for mAbs.
- Simulated a 4-column continuous process with LFG.
Main Results:
- Achieved a two-fold increase in sample loading productivity compared to constant flow-velocity (CF) operation.
- Demonstrated a 13% reduction in resin cost per unit of processed mAbs in a simulated continuous process.
- Maintained a 1.4 times enhancement in productivity with LFG compared to CF in the continuous process.
- Observed more pronounced efficiency improvements with favorable isotherms and large loading volumes.
Conclusions:
- The LFG method offers a significant improvement in capture chromatography process efficiency.
- LFG provides a viable strategy for enhancing productivity and reducing purification costs for mAbs.
- The developed parameter determination method enables the design of optimal LFG protocols for various chromatographic conditions.
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