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FRET-based fluorometry assay for curcumin detecting using PVP-templated Cu NCs
Zhixia Yao1, Hanmeng Liu1, Yaosheng Liu1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China.
Talanta
|December 10, 2020
Summary
This study introduces a new fluorescent method using polyvinyl pyrrolidone-templated copper nanoclusters (PVP-Cu NCs) for sensitive curcumin detection. The technique offers a reliable way to quantify curcumin in various samples with high selectivity.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Curcumin, a natural compound, has various health benefits but requires accurate quantification.
- Developing sensitive and selective detection methods for curcumin is crucial for its applications.
Purpose of the Study:
- To develop a novel fluorometric strategy for curcumin detection.
- To utilize polyvinyl pyrrolidone-templated copper nanoclusters (PVP-Cu NCs) as a fluorescent probe.
Main Methods:
- Fabrication of PVP-Cu NCs as a fluorescent probe.
- Investigating the interaction between PVP-Cu NCs and curcumin using fluorescence spectroscopy.
- Analyzing the fluorescence quenching mechanism, identified as fluorescence resonance energy transfer (FRET).
Main Results:
- PVP-Cu NCs exhibited distinct excitation/emission peaks at 380/510 nm.
- Curcumin caused fluorescence quenching of PVP-Cu NCs due to FRET.
- The method achieved a linear detection range of 0.1-10 μg mL⁻¹ with a detection limit of 21 ng mL⁻¹.
- The assay demonstrated good selectivity and was successfully applied to real samples.
Conclusions:
- The proposed PVP-Cu NCs-based fluorometric method is effective for sensitive and selective curcumin detection.
- This approach offers a promising tool for curcumin analysis in complex matrices.
