Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC₅₀, 0.016 µg/ml) followed by voriconazole (MIC₅₀, 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.