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Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device.

Pallavi Shinde1, Srabani Kar2, Mohan Loganathan1,3

  • 1Department of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, India.

ACS Biomaterials Science & Engineering
|December 15, 2020
PubMed
Summary
This summary is machine-generated.

Infrared laser pulses activate titanium microdish arrays for efficient parallel intracellular delivery. This novel method enables rapid, high-viability delivery of biomolecules into millions of cells.

Keywords:
Ti microdishcell viabilitydelivery efficiencyinfrared light pulse irradiationparallel intracellular deliverytransient heating

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Efficient intracellular delivery of biomolecules is crucial for research and therapeutics.
  • Existing methods often face limitations in scalability, efficiency, and cell viability.
  • Novel platforms are needed to overcome these challenges for broad applications.

Purpose of the Study:

  • To develop and demonstrate a highly efficient, parallel intracellular delivery platform using infrared (IR) pulse laser-activated titanium microdish (TMD) devices.
  • To investigate the efficacy of this platform for delivering various biomolecules, including dyes and dextrans of different molecular weights, into diverse cell types.
  • To optimize laser parameters for maximal delivery efficiency and cell viability.

Main Methods:

  • Fabrication of a two-dimensional array of titanium microdish (TMD) devices.
  • Activation of TMD devices using IR laser pulse irradiation to generate photothermal cavitation bubbles.
  • Disruption of cell membranes to create transient pores for biomolecule diffusion.
  • Delivery of propidium iodide dye and dextrans (3000 MW and 10,000 MW) into SiHa cells and other cell types.
  • Optimization of laser pulse parameters (number of pulses) to achieve high delivery efficiency and cell viability.

Main Results:

  • Successful parallel intracellular delivery of dyes and dextrans into various cell types.
  • Demonstrated high throughput, transfecting over a million cells within a minute.
  • Achieved 96% delivery efficiency and 98% cell viability for propidium iodide dye in SiHa cells using 600 pulses.
  • Obtained 98% delivery efficiency and 100% cell viability for 3000 MW dextran using 700 pulses.
  • Reported 92% delivery efficiency and 98% cell viability for 10,000 MW dextran.

Conclusions:

  • The IR laser-activated TMD device offers a highly efficient and parallel method for intracellular delivery.
  • The platform demonstrates excellent delivery efficiency and cell viability across different biomolecules and cell types.
  • This compact and user-friendly technology holds significant potential for applications in cellular therapy and diagnostics.