[Lipolytic activity of Mycobacterium rubrum cells, immobilized in polyacrylamide gel]

The cells of Mycobacterium rubrum 403 possessing lipolytic activity can be immobilized in polyacrylamide gel. The lipolytic activity of immobilized cells is 20-30 per cent of the activity of free cells. The lipolytic activity of immobilized cells does not changee after they have been used once and then stored at +4 degrees C during 5 months. If immobilized cells have been used several times, their lipolytic activity does not change during 1.5 months. In the case of immobilized cells, the temperature optimum is broader, pH is more alkaline, and the inhibiting action of Mg and Cu ions decreases.